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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/12/2020 |
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Actualizado : |
11/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; LOPEZ, V.; TAVARES, E.; SANTIÑAQUE, F.; DALLA RIZZA, M. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VICTOR JULIAN LOPEZ DEL PUERTO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. TAVARES, Forestal Oriental, Paysandú Uruguay.; F. SANTIÑAQUE, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Polyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs]. |
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Complemento del título : |
Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Agrociencia Uruguay 2020, v. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413 |
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Páginas : |
9 p. |
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ISSN : |
2301-1548 |
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DOI : |
10.31285/AGRO.24.381 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). |
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Contenido : |
Eucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii. |
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Palabras claves : |
COLCHICINA; COLCHICINE; CULTIVO IN VITRO; EUCALIPTO; IN VITRO CULTURE; ORYZALIN; ORYZALINE; POLIPLOIDIA; POLYPLOIDY. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14914/1/Castillo-Agrociencia-2020.pdf
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Marc : |
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024 7 $a10.31285/AGRO.24.381$2DOI
100 1 $aCASTILLO, A.
245 $aPolyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs].$h[electronic resource]
260 $c2020
300 $a9 p.
500 $aArticle history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020).
520 $aEucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii.
653 $aCOLCHICINA
653 $aCOLCHICINE
653 $aCULTIVO IN VITRO
653 $aEUCALIPTO
653 $aIN VITRO CULTURE
653 $aORYZALIN
653 $aORYZALINE
653 $aPOLIPLOIDIA
653 $aPOLYPLOIDY
700 1 $aLOPEZ, V.
700 1 $aTAVARES, E.
700 1 $aSANTIÑAQUE, F.
700 1 $aDALLA RIZZA, M.
773 $tAgrociencia Uruguay 2020$gv. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413
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INIA Treinta y Tres (TT) |
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| Registros recuperados : 5 | |
| 2. |  | PEREIRA, L.S. Necessidades de água e de rega das culturas : uso de modelos. In: GRUPO DE DESARROLLO DE RIEGO; SEMINARIO INTERNACIONAL, 1ER. AGOSTO 2010, PAYSANDÚ, URUGUAY. Potencial del riego extensivo en cultivos y pasturas. Montevideo (Uruguay): INIA, 2010. p. 73-90 Bibliografía: p. 198-200| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | SOUZA CONGIO, G. F. DE; BANNINK, A.; MAYORGA, MOGOLLÓN, O. L.; NICOLOV HRISTOV, A.; JAURENA, G.; GONDA, H.; GERE, J. I.; CERÓN-CUCCHI, M.E.; ORTIZ-CHURA, A.; TIERI, M.P.; HERNÁNDEZ, O.; RICCI, P.; JULIARENA, M.P.; LOMBARDI, B.; ABDALLA, A.L.; ABDALLA-FILHO, A.L.; BERNDT, A.; ANCHAO OLIVEIRA, P. P.; HENRIQUE, F. L.; GOMEZ MONTEIRO, A.L.; BORGES, L. I.; RIBEIRO-FILHO, H.M.N.; RIBEIRO PEREIRA, L.G.; RIBEIRO TOMICH, T.; MAGALHAES CAMPOS, M.; SAMARINI MACHADO, F.; MARCONDES, M. I.; ZERLOTTI MERCADANTE, M. E.; SANNOMIYA SAKAMOTO, L.; GALVAO ALBUQUERQUE, L.; FACCIO CARVALHO, P. C. DE; ROSSETTO, J.; SAVIAN, J.V.; MAZZA RODRIGUES, P. H.; PERNA JÚNIOR, F.; MOREIRA, T.S.; MAURÍCIO, R. M.; PACHECO RODRIGUES, J.P.; CRUZ BORGES, A.L. DA C.; REIS E SILVA, R.; FERREIRA LAGE, H.; ANDRADE REIS, R.; RUGGIERI, A.C.; CARDOSO, A. DA SILVA; SILVA, S. CARNEIRO DA; BARBOSA CHIAVEGATO, M.; VALADARES-FILHO, S. DE CAMPOS; SILVA, F. A. DE SALES; ZANETTI, D.; BERCHIELLI, T.T.; DUARTE MESSANA, J.; MUÑOZ, C.; ARIZA-NIETO, C.J.; SIERRA-ALARCÓN, L.I.; GUALDRÓN-DUARTE, L.B.; MESTRA-VARGAS, L.I.; MOLINA-BOTERO, I. C.; BARAHONA-ROSALES, R.; ARANGO, J.; GAVIRIA-URIBE, X.; GIRALDO VALDERRAMA, L.A.; ROSERO-NOGUERA, J.R.; POSADA-OCHOA, S.L.; ABARCA-MONGE, SERGIO; SOTO-BLANCO, R.; KU-VERA, J.C.; JIMÉNEZ-OCAMPO, R.; FLORES-SANTIAGO, E. DEL J.; CASTELÁN-ORTEGA, O.A.; VÁZQUEZ-CARRILLO, M.F.; BENAOUDA, M.; GÓMEZ-BRAVO, C. A.; ALVARADO BOLOVICH, V.I.; DÍAZ CÉSPEDES, M.A.; ASTIGARRAGA, L. Enteric methane mitigation strategies for ruminant livestock systems in the Latin America and Caribbean region: a meta-analysis. Journal of Cleaner Production. 2021. v. 312, art. 127693, 13 p. Doi: https://doi.org/10.1016/j.jclepro.2021.127693 Article history: Receiived 22 December 2020; Received in revised form 24 April 2021; Accepted 25 May 2021. Available online 30 May 2021.
Latin America Methane Project Collaborators está integrada por 71 investigadores en este artículo.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : -- - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| Registros recuperados : 5 | |
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